Avaliação de primers universais bacteriano em PCR in silico
DOI:
https://doi.org/10.24863/rib.v9i2.124Abstract
As técnicas de biologia molecular são indispensáveis em diversas áreas de pesquisa, dentre elas está a identificação de espécies bacterianas como Staphylococcus aureus, Streptococcus pneumoniae, Pseudomona auruginosa, Klebsiella pneumoniae, Corynebacterium diphtheriae, Mycobacterium tuberculosis e Escherichia coli e para isso utilizam primers universais. Este trabalho busca avaliar a qualidade deste primers universais em amplificar e diferenciar as bactérias descritas. Para isso foi utilizado uma PCR in silico e observados os resultados das amplificações. As amplificações demonstraram uma redundância ou mesmo ineficiência de vários dos primers testados.
Palavras-chave: Primers; Bacterias; PCR in silico.
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